The game, which kicked-off at 12noon Irish time in Pratelstvi Stadium in the Czech Republic, ended in a 4-0 win for the USA side.
Irish squad manager Sue Ronan made five changes to the squad that started Tuesday’s 4-0 win over Belgium in the first game of the friendly tournament.
As with that fixture, all 20 players in the squad took part in the game, in line with the rules of the tournament.
Speaking after the game, Ronan explained that the USA have an extremely strong side, with a lot of quality players.
She said: “They are a very good side, they are powerful, strong, fit – everything you are looking for in a squad. It also took us a while to get into the game, there was an element of fatigue in our side from the first match.
“We just didn’t weather the storm properly the way we did against Belgium. But the players aren’t used to these sorts of situations, that’s the main aim of this tournament and they will be learning all the time.”
And Ronan added that there were encouraging signs for the young Irish side.
“There were positives for us, they dug deep and never gave up. The possession stats were positive for us, they were about 50 to 40 per cent. We now have the Czech Republic on Saturday and we’ll be looking forward to that game,” explained Ronan.
Republic of Ireland: Rugile Askalnyte, Therese Kinnevey, Shauna Brennan, Jessica Stapleton, Della Doherty (pictured), Aoibheann Clancy, Kerryanne Brown, Erin McLaughlin, Ellen Molloy, Rebecca Watkins, Muireann Devaney.
Substitutes: Emma Ring, Chellene Trill, Kate O'Dowd, Zoe Leonard, Hazel Cawley, Teegan Lynch, Emer O'Neill, Aoife Horgan, Anna Fahey.
USA: Jordyn Gunnarson, Macy Blackburn, Maddie Mooney, Korbin Albert, Gio Costa, Layne St. George, Josie Master, Phoenix-Dawn Miranda, Emily Colton, Karlie Lema, Bella Sember.
Substitutes: Carolyn Calzada, Aidan McConnell, Simi Awujo, Sophie Fisher, Riley Gleason, Makhiya McDonald, Haley Craig, Carina Lageyre, Summer Denigan.
UEFA Women's Under-16 Development Tournament
May 21, Republic of Ireland 4-0 Belgium 13:30 CET, Pratelstvi Stadium
May 23, Republic of Ireland 0-4 USA 13:00 CET, Pratelstvi Stadium
May 25, Czech Republic v Republic of Ireland 11:00 CET, Rosického Stadium